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BIO-3318: Methods in biotechnology Work Load
10 credits (ECTS) | Type of course
Theoretical and practical. | Level of course/Year of study
First or second year at master''s level. | Language of instruction
Norwegian or English | Examination semester
Spring | Objectives
The students should master theoretical and technical approaches needed to construct a gene library, to identify and sub-clone a gene for a known protein and attain good gene expression in an appropriate vector and host. | Contents
The course is particularly aimed at master students of technology and covers the first step in a typical closed biotechnological process to produce a recombinant product. The lab work which forms the greater part of the course, will make use of recombinant DNA technology to clone and express the gene which codes for a known natural protein. The first part of the task will be to construct a gene library (cDNA/genomic). The next step is to identify and characterise the gene that codes for the known protein, and finally the gene will be sub-cloned in an appropriate expression vector that will yield good gene expression in a suitable host. The known natural protein may be from a eukaryotic (e.g. plant, fish, micro-organism) or prokaryotic organism. Bioinformatics will be an important instrument in this work. The lectures will cover relevant topics of recombinant DNA technology / biotechnology and also explain how lab records and reports should be compiled. | Recommended previous qualifications
BIO-1001 Cell and molecular biology, BIO-2302 Molecular cellbiology, BIO-2303 Genetics, BIO-2304 Introduction to Bioinformatics | Form of teaching
Lectures, student colloquia / seminar and compulsory lab work | Exam and assessment
Submitted lab reports
Grade type: pass / fail
| Contact person/lecturer
Student Advisor Karin Eilertsen, tlf.: 77 64 46 09
| Recommended reading/syllabus
Available on request | Course overlap
BIO-352S -1 9 sp |
Teaching spring 2006
Første fremmøte er obligatorisk. Undervisningen går intensivt i uke 3-9. Praktisk informasjon om emnet blir gitt på første fremmøte. Undervisningen kan bli gitt på engelsk. Ansvarlig institutt: Institutt for medisinsk biologi.
Første fremmøte: 20.01.06 kl. 10.15 på Aud.4
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