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BIO-3134: Applied Molecular Genetics Work Load 20 credits (ECTS) | Type of course Theoretical and practical subject. | Level of course/Year of study Master level course. | Language of instruction Norwegian or English by request. | Examination semester Autumn
| Contents The aim with this course is to give a thorough introduction to molecular genetic methods used in modern biological research. All lectures will be closely connected to the practical problems to be solved in the laboratory. Written laboratory reports will be subjects for plenary discussions as well as one obligatory literature summary. The course will give a basis for advanced studies in different areas of microbiology and will therefore contain problems concerning medicine, ecology and food-microbiology. Four practical problems are planned within the following topics: Taxonomy, genetic diversity, identification and characterisation, and horizontal gene transfer. The following methods will be used: rep-PCR-fingerprinting, 16S-RFLP, in situ hybridisation, DGGE (denaturing gradient gel electrophoresis), Pulsed field gel electrophoresis (PFGE), conjugation and transformation. The teaching will also address phylogeny and microarray technology.
| Recommended previous qualifications BIO-1001 Cell and molecular biology, BIO-2601 General microbiology | Form of teaching Lectures 10 hours, field and laboratory exercises 60 hours, seminars and project work 40 hours. | Exam and assessment Laboratory report, seminars based on submitted written material from group work, graded project assay, 4 h written exam with grades. Letter grades, A-F. | Contact person/lecturer Anne Høydal, e-post : anne.hoydal@ib.uit.no, tlf: 77 64 43 89 eller Cecilie Andreassen, cecilie.andreassen@ib.uit.no, tlf: 77 64 63 81 | Recommended reading/syllabus Available on request | Course overlap BIO-340 -1 15 sp BIO-8104 -1 20 sp |
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