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BIO-3300: Methods in biochemistry Work Load
10 credits (ECTS) | Type of course
Theoretical and practical. | Level of course/Year of study
First or second year at master´s level. | Language of instruction
Norwegian or English | Examination semester
Autumn
| Objectives
The course should give the student skills and insight into state of the art biochemical methods for biological and medical research. | Contents
This practical course revolves around four main topics that are explored using a spectrum of biochemical methods. After introductory lectures the laboratory work is carried out and the results are discussed in follow-up seminars. Topics for the exercises are: 1. Immunochemical analysis of immunoglobulins in egg yolk and of transferrin in blood using immunodiffusion, immunoelectrophoresis, miniature western blotting and EIA. 2. Highlighting structural and functional aspects of the blood clotting protein prothrombin, using affinity chromatography, electrophoresis and western blotting, and also comparative analysis of blood platelets and microparticles using 2D-electrophoresis. 3. Enzyme kinetical studies of alcohol dehydrogenase from yeast, using active site titration, velocity assay, inhibition and inactivation techniques, as well as group modifications. 4. Purification and binding studies of a transcription factor from zebra fish, expressed as a fusion protein in E.coli, using affinity purification (exploiting the fusion part), PCR, electrophoresis and gel retardation assay.
| Compulsory prerequisites
BIO-1001 Cell and molecular biology | Form of teaching
16 hrs of lectures, 16 hrs of seminars, 88 hrs of lab work. The lab exercises are compulsory. | Exam and assessment
Evaluation of lab reports.
Grade type: pass/ fail
| Contact person/lecturer
Student Advisor Karin Eilertsen, tlf.: 77 64 46 09 | Recommended reading/syllabus
Compendium. Recommended reading: Principles and Techniques of Biochemistry and Molecular Biology by K Wilson and J Walker, (Cambridge University Press, Cambridge, 2005, 802 pp., ISBN 0-521-82889-9 | Course overlap
BIO-352 -1 10 sp
BIO-352AS -1 6 sp | Homepage
http://uit.no/medfak/
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