http://hdl.handle.net/1956/933 Search the Channel search http://bora.uib.no/simple-search http://hdl.handle.net/1956/2370 Title: Investigation of tumour characteristics and treatment strategies in animal models using multiparametric MRI and novel contrast agents <br/> <br/>Authors: Rygh, Cecilie Brekke Wed, 20 Jun 2007 22:58:59 GMT http://hdl.handle.net/1956/2208 Title: Hyperoxia retards growth and induces apoptosis and loss of glands and blood vessels in DMBA-induced rat mammary tumors <br/> <br/>Authors: Raa, Anette; Stansberg, Christine; Steen, Vidar M.; Bjerkvig, Rolf; Reed, Rolf K.; Stuhr, Linda E. B. <br/> <br/>Abstract: Background: This study investigated the effects of hyperoxic treatment on growth, angiogenesis, apoptosis, general morphology and gene expression in DMBA-induced rat mammary tumors. Methods: One group of animals was exposed to normobaric hyperoxia (1 bar, pO2 = 1.0 bar) and another group was exposed to hyperbaric hyperoxia (1.5 bar, pO2 = 1.5 bar). A third group was treated with the commonly used chemotherapeutic drug 5- Fluorouracil (5-FU), whereas animals housed under normal atmosphere (1 bar, pO2 = 0.2 bar) served as controls. All treatments were performed on day 1, 4, 7 and 10 for 90 min. Tumor growth was calculated from caliper measurements. Biological effects of the treatment, was determined by assessment of vascular morphology (immunostaining for von Willebrandt factor) and apoptosis (TUNEL staining). Detailed gene expression profiles were obtained and verified by quantitative rtPCR. Results: Tumor growth was significantly reduced (~57–66 %) after hyperoxic treatment compared to control and even more than 5-FU (~36 %). Light microscopic observations of the tumor tissue showed large empty spaces within the tissue after hyperoxic treatment, probably due to loss of glands as indicated by a strong down-regulation of glandular secretory proteins. A significant reduction in mean vascular density (30–50%) was found after hyperoxic treatment. Furthermore, increased apoptosis (18–21%) was found after hyperoxic treatment. Conclusion: Thus, by increasing the pO2 in mammary tumor tissue using normobaric and moderate hyperbaric oxygen therapy, a significant retardation in tumor growth is achieved, by loss of glands, reduction in vascular density and enhanced cell death. Hyperbaric oxygen should therefore be further evaluated as a tumor treatment. Mon, 29 Jan 2007 22:58:59 GMT http://hdl.handle.net/1956/2138 Title: Expression of biomarkers (p53, transforming growth factor alpha, epidermal growth factor receptor, c-erbB-2/neu and the proliferative cell nuclear antigen) in oropharyngeal squamous cell carcinomas <br/> <br/>Authors: Ibrahim, Salah Osman; Lillehaug, Johan R.; Johannessen, Anne Christine; Liavaag, P. G.; Nilsen, Rune; Vasstrand, Endre N. <br/> <br/>Abstract: Using immunohistochemistry, expression of p53, transforming growth factor-alpha (TGF-α), epidermal growth factor receptor (EGFR), c-erbB-2/neu and proliferating cell nuclear antigen (PCNA) was examined in 26 fresh frozen tissue specimens of oropharyngeal squamous cell carcinomas (SCCs). p53 gene mutations were examined by polymerase chain reaction (PCR)/DNA sequencing methods in 22 carcinomas. The findings were examined for correlations with patients’ clinicopathological parameters. Expressions of p53 and PCNA were also examined in 21 formalin-fixed corresponding tissues. Of the fresh frozen tissue specimens, 77% (20/26) showed expression and 68% (15/22) showed mutations (substitutions) of the p53, with significant clustering of the mutations in exons 5 (8/22; 36%), 7 (4/22; 18%) and 8 (5/22; 23%). No mutations were found in exon 6. There was a discordance between expression of p53 protein and mutations of the gene. Parallel to expression and mutations of the p53 found in most of the specimens, expression of TGF-α, EGFR, c-erbB-2/neu and PCNA was found in 88% (22/25), 92% (23/25), 58% (14/24) and 91% (21/23) of the specimens, respectively. For the formalin-fixed tissue specimens, 62% (13/21) and 90% (19/21) expressed p53 and PCNA, respectively. Examining for correlations with patients’ clinicopathological parameters, expression of p53, TGF-α, EGFR and c-erbB-2/neu seemed to negatively correlate with the increase of the tumour grade. The present work suggests that: (1) lack of negative growth regulation due to inactivation of the p53 gene together with activation of other proto-oncogenes are necessary genetic events in the carcinogenesis of oropharyngeal SCCs; (2) in oropharyngeal SCCs, p53 gene mutations were clustered in exons 5 (codons 130–186), 7 (codons 230–248) and 8 (codons 271–282) which perhaps suggests that tobacco carcinogens probably affect the mutational hot spots of the p53 gene at codons 157, 175, 186, 248, 273 and 282; and (3) fresh frozen and formalin-fixed tissue specimens give similar results when an immunohistochemical method is applied. The importance of p53, TGF-α, EGFR, c-erbB-2/neu and PCNA as biomarkers in oropharyngeal SCCs deserves particular attention because it might offer further understanding of the development of these carcinomas. Thu, 29 Oct 1998 22:58:59 GMT http://hdl.handle.net/1956/2097 Title: Studies on viral gene transfer and angiogenesis inhibition in experimental glioma models <br/> <br/>Authors: Huszthy, Peter Csaba Thu, 02 Nov 2006 22:58:59 GMT http://hdl.handle.net/1956/2096 Title: Cognitive and psychosocial problems in children with epilepsy : a population-based approach <br/> <br/>Authors: Høie, Bjørg Thu, 11 Jan 2007 22:58:59 GMT http://hdl.handle.net/1956/2013 Title: Chronic mild stress - an anmial model of depression : from behavior to molecules <br/> <br/>Authors: Grønli, Janne Thu, 02 Nov 2006 22:58:59 GMT http://hdl.handle.net/1956/1507 Title: Expression and effects of IL-1 and TNF-α in different experimental models of dental inflammation <br/> <br/>Authors: Bletsa, Athanasia <br/> <br/>Description: List of publications This thesis is based on the following papers, which will be referred to by their roman numerals throughout the text: I. Bletsa A, Heyeraas KJ, Haug SR, Berggreen E (2004): “ IL-1α and TNF-α expression in rat periapical lesions and dental pulp after unilateral sympathectomy”, Neuroimmunomodulation 11: 376-384. II. Bletsa A, Nedrebø T, Heyeraas KJ, Berggreen E (2006): “Edema in Oral Mucosa after LPS or Cytokine Exposure”, Journal of Dental Research 85(5): 442-446. III. Bletsa A, Berggreen E, Fristad I, Tenstad O, Wiig H (2006): “Cytokine signalling in rat pulp interstitial fluid and transcapillary fluid exchange during LPS-induced acute inflammation”, Journal of Physiology 573: 225- 236. IV. Bletsa A, Berggreen E, Brudvik P: “IL-1α and TNF-α expression during early phases of experimental orthodontic tooth movement”, European Journal of Oral Sciences In press. Mon, 03 Jul 2006 22:58:59 GMT http://hdl.handle.net/1956/1364 Title: In vivo mapping of tumor microcirculation parameters using dynamic contrast enhanced MR imaging <br/> <br/>Authors: Stålsett, Eli Wed, 29 Oct 2003 22:58:59 GMT