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The National Institute of Nutrition and Seafood Research (NIFES) >
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http://hdl.handle.net/1956/2520
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| Tittel: | Application of PCR and DGGE to characterise the microflora of farmed fish |
| Forfattere: | Hovda, Maria Befring |
| Dato: | 9-Nov-2007 |
| Forlag: | The University of Bergen |
| Sammendrag: | Farming of fish has become an increasingly important part of the Norwegian fishing
industry. Furthermore, the Norwegian fishing sector represents one of the largest
national industries in terms of economic value. Fish is a perishable product and a
suitable substrate for chemical degradation and bacterial growth. Determinations of
shelf-life of fish and fish products have traditionally been based on microbial,
chemical and sensory evaluation. It is, however, important to have methods for a
rapid and reliable assessment of the microflora, and to aid in the determination of the
shelf-life of the fish products. This thesis discusses and describes the use of PCR and
denaturing gradient gel electrophoresis (DGGE) to detect and characterise the
microflora of farmed Atlantic halibut and cod. The aims were to introduce and apply
molecular biological methods for characterisation of the microflora, and to use these
methods to detect the changes in the microflora as a function of shelf-life extending
treatments. An additional aim was to compare the results obtained from molecularbased
and culture-based methods.
The DGGE and subsequent sequencing approach displayed the bacterial flora of the
farmed fish, and identified the predominant microflora. When applying the
sequencing approach, Photobacterium spp., Pseudomonas spp., Brochothrix
thermosphacta, Serratia sp., Yersinia sp., Micrococcus luteus and Shewanella spp.
were found to be the predominant bacteria in farmed Atlantic cod and halibut, stored
under modified atmosphere (MA). The method detected a more diverse bacterial flora
than previously obtained when culture-based methods were applied.
Bacterial DNA extracted directly from the sample, without prior cultivation, gave a
more diverse bacterial community. Furthermore, the molecular methods have been
used to study the effects of MA packaging and ozone treatment on the microflora
composition. There was no observable effect of ozone treatment of farmed cod. |
| URI: | http://hdl.handle.net/1956/2520 |
| ISBN: | 978-82-308-0413-1 (print version) |
| Vises i samlingene: | The National Institute of Nutrition and Seafood Research (NIFES)
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