Basic Course in Flow Cytometry

Credits (ECTS)

5

Aim and Content

Aim: Basic introduction to flow cytometry. The practical part of the course should enable the students to understand applications for flow cytometry. In addition, the applications used during the practical part of the course will be introduced in seminars by the course leaders.

Content:

Theoretical part:

• The course leaders will give a theoretical introduction to flow cytometry and some of the most common applications.

Practical part: Hands-on training at one of the local flow cytometers (BD accuri)

• • Quantification of apoptotic cells
  • Cell cyclus analyses
  • Simple staining of cells with surface markers
  • Analysis of transfected cells

Learning Outcomes

Knowledge

Upon completing the course, the participants should

- be familiar with basic applications for flow cytometry

- know which and how many different fluorochromes one can use on a given flow cytometer

- be able to evaluate the quality of flow cytometry data

Skills

Upon completing the course, the participants should be able to

- plan and perform basic experiments using flow cytometry

- quality control generated flow cytometry data

- present flow cytometry data in a scientific way

Competence

Upon completing the course, the participants should

- have basic understanding of flow cytometry

- understand why to use flow cytometry in a certain research question

- be able to interpret basic flow cytometry data

Course offered (semester)

Spring

The course will only be given if at least 4 students register for the  course, and is limited to 10 students.

Language of Instruction

English

Course Unit Level

Master students, PhD candidates

Department

Department of Clinical Science

Pre-requirements

Basic knowledge on apoptosis and cell cycle

Teaching Methods

23 x 1 hour lectures/seminars, 17 x 1 hour practical lab work

The course will only be given if at least 4 students register for the  course, and is limited to 10 students.

Assessment methods

Written lab report (composed of Introduction, Materials and Methods,

Results, Discussion). Has to be approved by the course leader.

Grading Scale

Passed / failed

Reading List

Flow cytometry - A Basic Introduction, Wiki version;

Michael Ormerod.

Place of Teaching

Department of Clinical Science.

Course Unit Evaluation

via MY Space

Contact Information

Silke Appel Phone: 559 74633, Silke.Appel@k2.uib.no

Marianne Enger Phone: 559 74660, Marianne.Enger@k2.uib.no

eller Studie@kliniskmedisin.uib.no