Blast2go
From Vd
Contents |
Blast2GO
Blast2GO (B2G) is a tool for functional annotation and analysis of gene or protein (uncharacterized) sequences. The tool was originally developed to provide a user-friendly interface for Gene Ontology annotation. Recent improvements have considerably increased the annotation functionality of the tool and currently Enzyme code, KEGG Maps and InterPro motifs are also supported.
Basically, B2G uses Blast searches to find similar sequences to one or several input sequences. The program extracts the GO terms associated to each of the obtained hits and returns an evaluated GO annotation for the query sequence(s). Enzyme codes are obtained by mapping from equivalent GOs while InterPro motifs are directly queried at the InterProScan web service. GO annotation can be visualized reconstructing the structure of the Gene Ontology relationships and ECs are highlighted on KEGG maps.
Input
Set of query sequences in Fasta format.
Output
The results are contained within an “.annot” file which can be opened in excel or a simple text editing program, Other useful information
A limit of 3000 sequences has been put on the input file.
Homepage
Contact persons
Ana Conesa: aconesa@cipf.es
Stefan Goetz: sgoetz@cipf.es
Citation
Ana Conesa, Stefan Götz, Juan Miguel García-Gómez, Javier Terol, Manuel Talón and Montserrat Robles Blast2GO: A universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics 2005 21: 3674-3676