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NARC - UiO
In Situ hybridization
In Situ protocols
Yeast Two-hybrid analysis
Yeast Two-hybrid protocols
 
 
www.narc.no
NARC - UMB
NARC - NTNU
 
 

Norwegian Arabidopsis Research Center

Please contact:
Reidunn B. Aalen
or
Ellen Andersen Department of Molecular Biosciences
Pb 1041, N-0316 Oslo, Norway.
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insitu hybridization, embryo     Arabidopsis flower     yeast
 
FUGE-logoS Norwegian Arabidopsis Research Center (NARC) is a national technology platform, sponsored by the Norwegian Functional Genomics initiative (FUGE), that will offer Norwegian plant scientists resources and technology in the Arabidopsis thaliana model system. NARC will establish a solid basis for basic plant molecular biology, applied plant research and plant breeding in Norway. The centre partners provide the basic technologies to utilize the powerful tools of functional genomics and thereby to promote the quality of plant science in Norway.
NARC is built on existing competence in Arabidopsis research at NTNU (Prof. Atle Bones), UiO (Prof. Reidunn Aalen) and NLH (Prof. Odd-Arne Rognli), and collaborates with other FUGE resource centres, e.g. The Norwegian Micro Array Consortium, imaging and proteomics facilities and bioinformatics expertise centres. By taking advantage of the tools already available, gene function and interaction of gene products can be studied in great detail.
At University of Oslo - NARC we focus on In situ hybridization for cellular localisation and expression analysis of specific transcripts, and Yeast two-hybrid analysis for protein-protein interactions and identification of binding domains and interacting/associated protein partners.

In Situ Hybridization

In situ hybridization is a useful technique for determining spatial patterns of gene expression. Fresh plant tissue is fixed, embedded in wax and sectioned. We use Roche DIG-labeling system for non-radioactive labeling of RNA probes for detection of the transcripts.
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Yeast Two-Hybrid Analysis

With the Yeast Two-hybrid technology we can screen cDNA libraries for novel proteins that interact with a known bait protein or DNA sequence, or confirm interactions between known proteins. We are using Clontechs Matchmaker Two-Hybrid Systems for both library construction and screening.
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News and NARC presentations

Poster representation at the 5th National Arabidopsis Meeting in Trondheim November 30, 2006.

NARC organized the 4th National Arabidopsis Meeting in Oslo, Norway December 8, 2005.

Poster representation at the 5th NorFA Workshop "Arabidopsis as a tool in molecular breeding" in Tune, Denmark, October 13-15, 2005.

Poster representation at The 16. International Conference on Arabidopsis Research in Madison, Wisconsin, USA, June 15-19, 2005.

NARC organized the 3rd National Arabidopsis Meeting and the 4th NorFA Workshop "Development, climatic adaptation and epigenetics" at Biri, Norway November 11-14, 2004.

NARC poster (pdf-format) presented at the 15. International Conference on Arabidopsis Research in Berlin, Germany July 11-14, 2004.

Visit our new site www.narc.no

06.02.04 - New design due to the release of the new web pages for Department of Molecular Biosciences.

NARC poster (pdf-format) presented at the 40th Biochemical Contact Meeting at Hafjell, january 15-18 2004.



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Last update 04.12.06 by Barbro E. Sæther