Toxicogenomics:

Toxicogenomics is changing the paradigm in investigative and predictive toxicology and target impacts of environmental stressors at the molecular and cellular levels through the application of state-of-the-art micro (macro) array technology in novel and innovative approaches. Prior to clinical manifestation, the first interaction between ESs and organisms occurs at the molecular and cellular levels. Changes in gene expression as a result of environmental stressors and the subsequent molecular processes that lead to adverse health effects may be used as quantitative marker (“biomarker”) for cellular, organismal and population effects. At the community level, environmental stressors are expected to lead to changes in species composition and abundance.

My laboratory is developing integrated genomics screening approach that accelerates the assessment of affected targets (e.g., receptors, proteins and other signal transduction molecules). Furthermore, the linkage between DNA response, mRNA transcription and protein expression resulting from contaminant stressors is essential in understanding mechanism of action, and hence effective mitigation. These are accomplished through the development of target gene chips for specific exposure conditions. Gene-environmental interactions are well documented, and such interactions can have significant impacts on individual and population viability. We have recently printed a 300-gene SalArray chip from salmon exposed to complex chemical mixtures. A related chip from Cod (CodArray) is in production for genomic evaluation of reproductive physiology in early oocyte growth.