Original Articles: Kusche-Gullberg, M.

1. Österholm, C., Lu, N., Lidén, Å., Karlsen, T.V., Gullberg, D., Reed R.K. and Kusche-Gullberg, M. Fibroblast EXT1-levels influence tumor cell proliferation and migration in composite spheroids. PLoS One 7(7):e41334 (2012)
   
   
2. Kusche-Gullberg, M., Nybakken, K., Perrimon, N. and Lindahl, U. Drosophila heparan sulfate, a novel design. Journal of Biological Chemistry 287(26):21950-21956 (2012)
   
   
3. Reine, T.M., Kusche-Gullberg, M., Feta, A., Jenssen, T. and Kolset, S.O. Heparan sulfate expression is affected by inflammatory stimuli in primary human endothelial cells. Glycoconjugate Journal 29(1):67-76 (2012)
   
   
4. Smeds, E., Feta, A. and Kusche-Gullberg, M. Target selection of heparan sulfate hexuronic acid 2-O-sulfotransferase. Glycobiology 20, 1274-1282 (2010)
   
   
5. Österholm, C., Barczyk, M.M., Busse, M., Grønning, M, Reed, R.K. and Kusche-Gullberg,  M. Mutation in the heparan sulfate biosynthesis enzyme EXT influences growth factor signaling and fibroblast interactions with the extracellular matrix. The Journal of Biological Chemistry 284, (50): 34935-34943 (2009)
   
   
6. Feta, A., Do, A.T., N., Rentzsch. F., Technau, U. and Kusche-Gullberg, M. Molecular analysis of heparan sulphate biosynthetic enzyme machinery and characterization of heparan sulphate structure in Nematostella vectensis. (2009) Biochemical Journal
   
   
7. Presto, J., Thuveson, M., Carlsson, P., Busse, M., Wilén, M., Eriksson, I., Kusche-Gullberg,  M. and Kjellén, L. Towards a GAGosome. Heparan sulfate biosynthesis enzymes EXT1 and EXT2 affect NDST1 expression and heparan sulfate sulfation. Proceedings of the National    Academy of Sciences U.S.A. 105 (12), 4751-4756 (2008)
   
   
8. Busse, M., Feta, A., Presto, J., Wilén, M., Grønning, M., Kjellén, L. and Kusche-Gullberg,  M. Contribution of EXT1, EXT2 and EXTL3 to heparan sulfate chain elongation. The Journal of Biological Chemistry 282, (45):32802-32810 (2007)
   
   
9. Langsdorf, A., Do, A.-T., Kusche-Gullberg, M., Emerson, C.P, Jr. and Ai, X. Sulfs are regulators of growth factor signaling for satellite cell differentiation and muscle regeneration. Developmental Biology 311, 464-477 (2007)
   
   
10. Whiteford, J.R., Behrends, V., Kirby, H., Kusche-Gullberg, M., Muramatsu, T. and Couchman, J.R. Syndecans promote integrin-mediated adhesion of mesenchymal cells in two distinct pathways. Experimental Cell Research 313, 3902-3913 (2007)
    
    
11. Ai, X., Kitazawa, T., Do, A.-T., Kusche-Gullberg, M., Labosky. P.A. and Emerson, C. P., SULF1 and SULF 2 regulate heparan sulfate-mediated GDNF signaling for esophageal innervation. Development 134(18):3327-3338 (2007)
    
    
12. Ledin, J., Ringvall, M., Thuveson,  M., Eriksson, I., Wilén, M., Kusche-Gullberg, M., Forsberg, E. and Kjellén, L. Enzymatically active N-deacetylase/N-sulfotransferase-2 is present in liver but do not contribute to heparan sulfate N-sulfation. The Journal of Biological Chemistry 281, 35727-35734 (2006)
    
    
13. Ai, X., Do, A.-T., Kusche-Gullberg, M., Lindahl, U., Lu, K. and Emerson, C. P., Jr. Substrate specificity and domain functions of extracellular heparan sulfate 6-O-endosulfatases, QSulf 1 and Qsulf2. The Journal of Biological Chemistry 281, 4969-4976 (2006)
    
    
14. Do, A.-T., Smeds. E., Spillmann, D. and Kusche-Gullberg, M. Overexpression of heparan sulfate 6-O-sulfotransferases in human embryonic 293 cells results in increased N-acetyl glucosaminyl 6-O-sulfation. The Journal of Biological Chemistry 281, 5348-5356(2006)
    
    
15. Bodevin-Authelet, S., Kusche-Gullberg, M., Pummill, P., DeAngelis, P. and Lindahl. U.  Biosynthesis of hyaluronan: direction of chain elongation. The Journal of Biological Chemistry 280, 8813-8818 (2005)
    
    
16. Xu, S., Zhao, L., Larsson, A., Smeds, E., Kusche-Gullberg, M., and Venge, P. Purification of a 75 kDa protein - N-acetylglucosamine-6-sulfatase from the organelle matrix of human neutrophils. Biochemical Journal 387, 841-847 (2005)
    
    
17. Lindahl, U., Li, J-p, Kusche-Gullberg, M., Salmivirta, M., Alaranta, S., Emeis, J., Marjamäki, A., Veromaa, T., Roberts, I., Taylor, C., Oreste, P., Zoppetti, G., Naggi, A., Torri, G., and Casu, B.    Generation of "Neoheparin" from E. coli K5 capsular polysaccharide.  Journal of Medicinal Chemistry. 48, 349-352 (2005)
    
    
18. Holmborn, K., Ledin, J., Smeds, E., Eriksson, I., Kusche-Gullberg, M. and Kjellén, L.  Heparan sulfate synthesized by mouse embryonic stem cells deficient in NDST1 and NDST2 is 6-O-sulfated but contains no N-sulfate groups. The Journal of Biological Chemistry 279, 42355-42358 (2004)
    
    
19. Yamada, S,, Busse, M., Ueno, M., Kelly, O.G., Skarnes, W.C., Sugahara, K., and Kusche-Gullberg, M. Embryonic fibroblasts with a gene trap mutation in Ext1 produce short heparan sulfate chains. The Journal of Biological Chemistry 279, 32134-32141 (2004)
    
    
20. Busse, M. and Kusche-Gullberg, M. In vitro polymerization of heparan sulfate backbone      structure by the EXT proteins. The Journal of Biological Chemistry 278, 41333-41337 (2003)
    
    
21. Roman, E., Roberts, I., Lidholt, K. and Kusche-Gullberg, M. Overexpression of UDP-Glucose Dehydrogenase in E coli results in decreased biosynthesis of K5 Polysaccharide.  Biochemical Journal 374, 767-772 (2003)
    
    
22. Ai, X., Do, A.-T., Lozynska. O., Kusche-Gullberg, M., Lindahl, U. and Emerson, C. P., Jr. QSulf1 remodels the sulfation states of cell surface heparan sulfate proteoglycans. The Journal of Cell Biology 162, 341-351(2003)
    
    
23. Jemth, P., Smeds, E., Do, A.-T., Habuchi, H., Kimata, K., Lindahl, U. and Kusche-Gullberg,  M. Oligosaccharide library-based assessment of heparan sulfate 6-O-sulfotransferase substrate specificity. The Journal of Biological Chemistry 278, 24371-24376 (2003)
    
    
24. Smeds, E., Habuchi, H., Do, A.-T, Hjertson, E., Grundberg, H, Kimata, K., Lindahl, U. and Kusche-Gullberg, M. Substrate specificities of mouse heparan sulfate glucosaminyl 6-O-sulfotransferases. Biochemical Journal 372, 371-380 (2003)
    
    
25. Habuchi, H., Miyake, G., Nogami, K., Kuroiwa, A., Matsuda, Y., Kusche-Gullberg, M., Habuchi, O., Tanaka, M. and Kimata, K.  Biosynthesis of heparan sulphate with diverse structures and functions: Two alternatively spliced forms of human heparan sulphate 6-O-sulphotransferase-2 having different expression patterns and properties. Biochemical Journal 371, 131-142 (2003)
    
    
26. Zhang, W.-M., Popova, S.N., Bergman, C., Velling, T., Kusche-Gullberg, M., and Gullberg, D. Analysis of the human integrin a11 gene (ITGA11) and its promoter.  Matrix Biology 21, 513-523 (2002)
    
    
27. Jemth, P., Kreuger, J., Kusche-Gullberg, M., Sturiale, L., Giménez-Gallego, G., and Lindahl. U. Biosynthetic oligosaccharides libraries for identification of protein-binding heparan sulfate motifs. The Journal of Biological Chemistry 277, 30567-30573 (2002)
    
    
28. Kim B.-T., Kitagawa, H., Tamura, J.-i., Kusche-Gullberg, M., Lindahl, U. and Sugahara, K. Demonstration of a novel gene DEXT3 of Drosophila melanogaster as the essential N-acetylglucosamine transferase in the heparan sulfate biosynthesis: chain initiation and elongation. The Journal of Biological Chemistry 277, 13659-13665 (2002)
    
    
29. Kim B.-T., Kitagawa, H., Tamura, J.-i., Saito, T., Kusche-Gullberg, M., Lindahl, U. and Sugahara, K. The human tumor suppressor EXT gene family members, EXTL1 and EXTL3 encode a1,4-N-acetylglucosaminyltransferases likely involved in heparan sulfate/heparin  biosynthesis. Proceedings of the National Academy of Sciences U.S.A. 98, 7176-7181 (2001)
    
    
30. Rong, J., Habuchi, H., Kimata, K., Lindahl, U. and Kusche-Gullberg, M. Substrate specificity of heparan sulfate hexuronyl 2-O-sulfotransferase. Biochemistry 40, 5548-5555 (2001)
    
    
31. Kitagawa, H. Egusa, N., Tamura, J.-i., Kusche-Gullberg, M., Lindahl, U. and Sugahara, K.  rib-2, a Caenorhabditis elegans homolog of the human tumor suppressor EXT genes encodes a novel a1,4-N-Acetylglucosaminyltransferase involved in the biosynthetic initiation and elongation of heparan sulfate. The Journal of Biological Chemistry 276, 4834-4838 (2001)
    
    
32. Kramerova, I. A., Kawaguchi, N., Fessler, L. I., Nelson, R. E., Chen, Y., Kramerov, A. A., Kusche-Gullberg, M., Kramer,  J. M., Ackley, B. D.,  Sieron, A. L., Prockop, D. J. and Fessler, J. H. Papilin in development; a pericellular protein with a homology to the ADAMTS metalloproteinase. Development 127, 5475-5485 (2000)
    
    
33. Senay, C., Lind, T., Muguruma K., Tone, Y., Kitagawa, H., Sugahara, K., Lidholt, K.,  Lindahl, U. and Kusche-Gullberg M.  The EXT1/ EXT2 tumor suppressors; catalytic activities and role in heparan sulfate biosynthesis. EMBO reports 1, 282-286 (2000)
    
    
34. Rong, J., Habuchi, H., Kimata, K., Lindahl, U. and Kusche-Gullberg, M. Expression of heparan sulphate L-iduronyl 2-O-sulphotransferase in human kidney 293 cells results in     increased D-glucuronyl 2-O-sulphation. Biochemical Journal 346, 463-468 (2000)
    
    
35. Velling, T., Kusche-Gullberg, M., Sejersen, T. and Gullberg, D. cDNA cloning and chromosomal localization of human  a11 integrin: a novel collagen-binding I-domain containing, ß1-associated integrin a-chain present in muscle tissues. The Journal of Biological Chemistry 274, 25735-25742 (1999)
    
    
36. Forsberg, E., Pejler, G., Ringvall, M., Lunderius, C., Tomasini-Johansson, B., Kusche-Gullberg, M., Eriksson, I., Ledin, J., Hellman, L. and  Kjellén, L. Abnormal mast cells in mice deficient in a heparin-synthesizing enzyme. Nature 400, 773-776 (1999)     
    
    
37. Lind, T., Falk, E., Hjertson, E., Kusche-Gullberg, M. and Lidholt, K. Molecular cloning and expression of UDP-glucose dehydrogenase from bovine liver. Glycobiology 9, 595-600 (1999)
    
    
38. Kusche-Gullberg, M., Eriksson, I., Sandbäck-Pikas, D. and Kjellén, L. Identification and expression in mouse of two heparan sulfate glucosaminyl N-deacetylase/N-sulfotransferase genes. The Journal of Biological Chemistry 273, 11902-11907 (1998)
    
    
39. Uhlin-Hansen, L., Kusche-Gullberg, M., Eriksson, I., Berg, E. and Kjellén, L. Mouse mastocytoma cells synthesize undersulfated heparin and chondroitin sulfate in the presence of brefeldin A. The Journal of Biological Chemistry 272, 3200-3206 (1997)
    
    
40. Nilsson, G., Blom, T., Harvima, I., Kusche-Gullberg, M., Nilsson, K. and Hellman, L. Stem cell factor-dependent human cord blood derived mast cells express a- and ß-tryptase, heparin and chondroitin sulfate. Immunology 88, 308-314 (1996)
    
    
41. Cheung, W.F., Eriksson, I., Kusche-Gullberg, M., Lindahl, U. and Kjellén, L. Expression of the mouse mastocytoma glucosaminyl N-deacetylase/N-sulfotransferase in human kidney 293 cells results in increased N-sulfation of heparan sulfate. Biochemistry, 35, 5250-5256 (1996)
    
    
42. van den Born, J., Gunnarsson, K., Bakker, M.A.H., Kjellén, L. Kusche-Gullberg, M., Maccarana, M., Berden, J.H.M. and Lindahl, U. Presence of N-unsubstituted glucosamine units in native heparan sulfate revealed by a monoclonal antibody. The Journal of Biological Chemistry 270, 31303-31309 (1995)
    
    
43. Nilsson, G., Blom, T., Kusche-Gullberg, M.,  Kjellén, L., Butterfield, J.H., Sundström, C., Nilsson, K. and Hellman, L. Phenotypic characterization of the human mast-cell line HMC-1.  Scandinavian Journal of Immunology 39, 489-498 (1994)
    
    
44. MacKrell, A.J., Kusche-Gullberg, M., Garrison, K., and Fessler, J.H. Novel Drosophila laminin A chain reveals structural relationships between laminin subunits. FASEB Journal  7, 375-381 (1993)
    
    
45. Kusche-Gullberg, M., Garrison, K., MacKrell, A.J., Fessler, L.I. and Fessler, J.H. Laminin A chain: expression during Drosophila development and genomic sequence. EMBO Journal 11, 4519-4527 (1992)
    
    
46. Pettersson, I., Kusche, M., Unger, E., Wlad, H., Nylund, L., Lindahl, U. and Kjellén, L. Biosynthesis of heparin. Purification of a 110 kDa mouse mastocytoma protein required for both glucosaminyl N-deacetylation and N-sulfation. The Journal of Biological Chemistry 266, 8044-8049 (1991)
    
    
47. Kusche, M., Oscarsson, L.-G., Reynertsson, R., Rodén, L. and Lindahl, U. Biosynthesis of heparin. Enzymatic sulfation of pentasaccharides. The Journal of Biological Chemistry 266,  7400-7409 (1991)
    
    
48. Kusche, M., Hannesson, H.H. and Lindahl, U. Biosynthesis of heparin. Use of Esherichia coli K5 capsular polysaccharide as a model substrate in enzymic polymer-modification reactions.   Biochemical Journal 275, 151-158 (1991)
    
    
49. Kusche, M., Torri, G., Casu, B. and Lindahl, U. Biosynthesis of heparin. Availability of glucosaminyl 3-O-sulfation sites. The Journal of Biological Chemistry 265, 7292-7300 (1990)
    
    
50. Kusche, M. and Lindahl, U. Biosynthesis of heparin. O-sulfation of  D-glucuronic acid units.  The Journal of Biological Chemistry 265, 15403-15409 (1990)
    
    
51. Kusche, M., Lindahl, U., Enerbäck, L. and Rodén, L. Identification of oversulfated galactosaminoglycans in intestinal-mucosal mast cells of rats infected with the nematode worm Nippostrongylus brasiliensis. Biochemical Journal 253, 885-893 (1988)
    
    
52. Kusche, M., Bäckström, G., Riesenfeld, J., Petitou, M. and Lindahl, U. Biosynthesis of heparin. O-sulfation of the antithrombin-binding region. The Journal of Biological Chemistry 263, 15474-15484 (1988)
    
    
53. Horner, A.A., Kusche, M., Lindahl, U. and Peterson, C.B. Determination of the range in binding-site densities of rat skin heparin chains with high binding affinities for antithrombin.  Biochemical Journal. 251, 141-145 (1988)
    
    
54. Enerbäck, L., , Kolset, S.O., Kusche, M., Hjerpe, A. and Lindahl, U. Glycosaminoglycans in  rat mucosal mast cells. Biochemical Journal 227, 661-668 (1985)
    
    
    Book chapters and review articles
    
    
55. Lindahl, U., Kusche, M., Lidholt, K. and Oscarsson, L.-G. Biosynthesis of heparin. Annals of the New York Academy of Sciences 556, 36-50 (1989)
    
    
56. Lindahl, U, Kusche-Gullberg, M. and Kjellén, L. Regulated diversity of heparan sulfate. The Journal of Biological Chemistry 273, 24979-24982 (1998)
    
    
57. Kusche-Gullberg, M. and Lindahl, U. Heparan Sulfate GlcA/GlcNAc Transferase. In,  Handbook of Glycosyltransferases and Related Genes (Eds. Naoyuki Taniguchi, Koichi Honke, Minoru Fukuda ) (2002)
    
    
58. Kusche-Gullberg, M and Kjellén L. Sulfotransferases in glycosaminoglycan biosynthesis.  Current Opinion in Structural Biology 13, 1-7 (2003)
    
    
59. Ai, X., Kusche-Gullberg, M., Lindahl, U. and Emerson, C. P., Jr. Remodeling of  heparan sulfation by extracellular endosulfatases. In “Chemistry and Biology of Heparin and Heparan Sulfate” (Ed. Garg, H.G) Elsevier Science Ltd, pp 245-258 (2006)